Chromogenic Technology

How does Chromogenic Culture Media technology work? 

 

This technology is based on soluble colourless molecules (called chromogens), composed of a substrate (targeting a specific enzymatic activity) and a chromophore.
When the target organism’s enzyme cleaves the colourless chromogenic conjugate, the chromophore is released. In its unconjugated form, the chromophore exhibits its distinctive colour and, due to reduced solubility, forms a precipitate.

 

 

 

Who invented this technology ?

The first chromogenic agar (for detection of  E.coli) was invented and patented by Dr. A. Rambach in 1979. The introduction of this medium triggered a revolution in microbial diagnosis and driven by the introduction of a whole range of media for the detection of key clinical & food borne pathogens. The use of chromogenic culture media for the detection of bacteria is increasing steadily despite the introduction of other (often molecular biology based) techniques. 

 

What are the advantages of this method?


FOR THE LABORATORY TECHNICIAN: EASY TO READ AT A GLANCE !

·       The result is very specific and distinctive.

·       colour-based differentiation method.

·       clearly distinguishable with the naked eye under normal lighting conditions. With this technique, colonies of specific microorganisms can be recognized by their colour at a glance.

Powerful colours on RambachTM Agar 


ECONOMIES OF SCALE: COST SAVINGS & FAST METHOD
 !

·       It allows for easy differentiation of microorganisms without the complex and costly traditional detection procedures employed in traditional agar testing techniques (No subcultures).

·       By saving time and labor, it increases the efficiency of laboratory testing.

·       By shortening the timeframe for identifying pathogens, it helps to prevent the spread of infections.

 

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